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KMID : 0374019930160040321
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Ewha Medical Journal
1993 Volume.16 No. 4 p.321 ~ p.330
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Effects on 5-HT and Ach in the Rat Brain by the I.C.V Injection of PCPA
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Abstract
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PCPA is drug which depletes serotonin by inhibiting tryptophan hydroxylase activity
Recent evidence indicates that the ascending cholinergic projection from the basal forebrain and ascending serotonergic projections from the brain stem exert a powerful control over the generalized electrical activity of the hippocampus formation
and
neocortex Cross & Deakin (1985) reported that destruction of ascending cholinergic neurons by injection of ibotenic acid into the basal forebrain results in a significant reduction in 5-HT1 receptor in the denervated cortex.
Maura & Raiteri (1986) observed that the release of (3H) Ach was concentration depenendently inhibited by exogenous 5-HT in rat hippocampus.
So, we were interested in investigating whether PCPA which depletes the serotonin also affects the cholinergic system. The brain (cortex area 3, 4, 17 and hippocampus) were exised on the 3day and 7day after single injection of PCPA 3mg/10μl into
the
cerebroventricle (Latefal ventricle AP+8.2mm ; L, 1.5mm ; DV+ 1.2mm).
5-HT and 5-HLAA contents were measured by HPLC-ECD and acetylcholine was measured by GC.
@ES The results are
@EN 1) 5-HT 5-HLAA contents in the cortex & hippocampus were decreased gradually after PCPA injection but not significant.
2) Ach contents in the cortex & hippocampus were decreased significantly on the 3rd day & 7th day after PCPA injection.
3) We observed that the cerebral cortex has small amount of vesicles or no vesicles and coated vesicles in the axon terminals by electren microscope.
So , these results suggest that PCPA treatment results in significant changes of cholinergic system in the brain.
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